AllFocusTM - Extended Depth of Field Software
The AllFocus software aligns images in the Z-stack by shifting, scaling, and rotating them and creates a composite image by choosing pixels from the best-focused plane. This produces an image where all parts are in focus, effectively increasing the depth of field. AllFocus software can work with images produced by compound and stereo microscopes, confocal microscopes, digital cameras with lenses, and other sources. It can be used with transmitted light, reflected light and fluorescence images. The AllFocus software can be used as a stand-alone application on a previously acquired Z-stack of images, or it can be controlled from other applications. This feature enables the use of AllFocus by system integrators. In particular, it can be used in conjunction with the ZStackTool image acquisition software, which controls Motorized Lift and Microscope Focus Drive. AllFocus provides the broadest available range of features for extending depth of field of images. See examples of processing of Z-stacks of images by AllFocus in the EDF Image Gallery. In addition to combining stacks of differently focused images, AllFocus can combine stacks of images acquired with with different exposure times (HDR Image Gallery) or at different angles of illumination (VAI Image Gallery).
Automatic alignment of images in Z-stack by optimizing scaling factor, shift, and rotation.
Automatic merging of best-focused areas of Z-stack images into a single image (best-focus source or weighted/exponentially weighted average).
Automatic correction of chromatic aberrations based on image content.
Supported file types: monochrome, RGB, and multispectral images.
Supported file formats: TIFF, JPEG, BMP.
Topographic map: calculation, display as grayscale, and writing to a file.
Parameters for aligning and merging are open for adjustment to the user (e.g., parameters of focus function, merge method, quality/speed settings, etc).
Can prioritize beginning or end of stack.
Control of AllFocus from external applications for easy software integration.
Inspection of original images and results:
slide show of selected images (original, aligned, or masks)
display of masks of pixels from each Z-plane selected for the merged image
display of contours of masks superimposed on the merged image
display of the source Z-plane number, coordinates, and value for the cursor position in the merged image
toggle with up and down arrows the merged image and the current Z-plane
switch with left and right arrows to the previous and to the next image in the Z-stack
choice of zoom and pan for viewing (displayed portion of the image is the same for all images in Z-stack).
Manual editing of merged image:
by copying pixels (drawing) from the selected source image (after alignment) into the result within a user-changeable cursor.
by eliminating connected components of the merge masks.
Download current version here. The program will work for 30 days with full functionality, after which a valid registration code will be required. You will receive the registration code by email after completing the purchase below.
Price: $50 for perpetual license.