AllFocusTM
EDF
Image Gallery -
Penicillium, Whole Mount
Whole Mount of
Penicillium culture from a slide made by
LiveBloodLabs
(www.livebloodlabs.com). Images were acquired on Olympus BX51 with UPlanFl 40X
0.75NA objective in transmitted light.
Condenser was set
at 0.75NA. Focus was motorized by the
Focus
Drive with Integrated Controller. Camera -
Canon Digital Rebel
positioned in primary image plane. Control software -
ZStackTool and
Canon Remote Capture. Z-stack
of 30 images was acquired with step size 1 micron and processed with
AllFocus V0.96.
Left image is the
Z-stack animation (not
all images from the stack are shown in
the animation)
and right image is the Merged Image produced by AllFocus.
In this case the
merge method was linear combination of images in the stack with weights
determined by focus function.
-F8S1N3A10-LinCon.jpg)