AllFocusTM EDF Image Gallery - Nylon Fibers
Images of Nylon Fibers were acquired on Olympus BX51 with UPlanFl 20X 0.50NA objective in transmitted light. Condenser was set at 0.3NA for higher contrast. Focus was motorized by the Focus Drive with Integrated Controller. Camera - Canon Digital Rebel positioned in primary image plane. Control software - ZStackTool and Canon Remote Capture. Z-stack of 47 planes was acquired with step size 1 micron and processed with AllFocus V0.95. In the animation below each third image is shown. Left image is the Z-stack animation and right image is the Merged Image produced by AllFocus.