AllFocusTM
EDF
Image Gallery - Diatom #3
Diatom Achinophychus Halionyx from an antique microscope slide prepared by W. Watson & Sons, London.
Images were acquired
on Olympus BX51 with UPlanFl 40X
0.75NA objective in transmitted light. Condenser was set at 0.4NA for
higher contrast. Diameter of the diatom is ~170
μm.
Focus was motorized by the
Focus
Drive with Integrated Controller. Camera -
Canon Digital Rebel
positioned in primary image plane. Control software -
ZStackTool and
Canon Remote Capture. Z-stack
of 20 planes was acquired with step size 0.5 microns and processed with
AllFocus V0.95.
Left image is the
Z-stack animation, middle image is the Merged Image produced by AllFocus. Right
image is the Height Map shown with standard color rendering where color
corresponds to height alternating with rendering by the Merged Image. (3D
rendering of the Height Map was done in
MATLAB)
