AllFocusTM EDF Image Gallery - Diatom #1
Diatom Asterolampra Vulgare from an antique microscope slide prepared by W. Watson & Sons, London. Images were acquired on Olympus BX51 with UPlanFl 40X 0.75NA objective in transmitted light. Condenser was set at 0.4NA for higher contrast. Diameter of the diatom is ~150 μm. Focus was motorized by the Focus Drive with Integrated Controller. Camera - Canon Digital Rebel positioned in primary image plane. Control software - ZStackTool and Canon Remote Capture. Z-stack of 24 planes was acquired with step size 0.5 microns and processed with AllFocus V0.95. Left image is the Z-stack animation, middle image is the Merged Image produced by AllFocus. Right image is the Height Map shown with standard color rendering where color corresponds to height alternating with rendering by the Merged Image. (3D rendering of the Height Map was done in MATLAB)